Expertise: Analyses of physiological and pathological importance and the molecular mechanisms of calcium and redox signaling, and their interplay, in the human skin and immune system. Detection and quantification of biologically-relevant oxidizing and reducing agents and enzymes. Determining physico-chemical (redox) properties of bio-molecules and their pharmacological potential.
Methodology provided: Sub-cellular measurements of calcium, H2O2, ATP, GSH and other cellular parameters using live-cell epifluorescent, FRET and TIRF microscopy. Functional assays essential for evaluation of skin and immune cell function. Detection of free radicals and oxygen consumption using EPR. Electrochemical techniques for evaluation of redox properties of bio-molecules and redox active enzymes. Primary human cell culture.