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Deponte, Marcel Heidelberg

Expertise: Comparative mechanistic characterization of redox enzymes and protein machineries from yeast, human and parasitic protists; enzymology; molecular parasitology; molecular evolution
Methodology provided: The Deponte lab provides experience regarding the mechanistic characterization of recombinant redox proteins from different eukaryotic lineages. This includes a variety of expression/purification systems, standardized spectrophotometric as well as gel-based redox assays and titrations, protein structure-function analyses using in silico modeling and site-directed mutagenesis as well as the characterization of enzyme inhibitors. In addition, the lab routinely uses reverse genetics and methods in molecular cell biology to compare the properties and functions of proteins in yeast, Leishmania tarentolae and Plasmodium falciparum.